MANNA METHOD (G. Mann, amer. the histologist, 1840 — 1912) — a way of histologic coloring for survey drugs and identification of fine cellular structures. It is offered in 1894. Apply M. of m also to identification of a neuroglia (see. Alzheimer methods of coloring ) and for coloring of little bodies of Negri at rage.
35 ml of 1% of solution of methylene blue mix with same (or up to 45 ml) the number of 1% of water solution of eosine and 100 ml of a distilled water. Cuts paint during 24 hours, wash out in water, dehydrate in absolute alcohol and process solution (4 drops of 1% of solution of caustic soda in absolute alcohol and 50 ml of absolute alcohol). After cuts in this solution redden, wash out them in pure absolute alcohol and transfer to water where they lose excess of paint. In 1,5 — 2 min. cuts transfer in slightly acidified acetic to - that water where they become blue again, and, having carried out through absolute alcohol and a xylol, conclude in balm. Cytoplasm is painted in violet color, chromatin, glial fibers — in dark blue, kernels and erythrocytes — in red. M of m apply both to smears, and to paraffin sections after sublimate or picric fixers.
See also Histologic methods of a research .
Bibliography: Lille R. Patogistologicheskaya of the technician and a practical histochemistry, the lane with English, page 548, M., 1969.