WRIGHT METHOD OF COLOURING

From Big Medical Encyclopedia

WRIGHT METHOD OF COLOURING (J. H. Wright, amer. the pathologist, 1871 — 1928) — one of kinds of a way of coloring of blood smears across Romanovsky. The river of m of the lake allows to reveal features of leukocytes quickly.

The method is offered in 1902 by Wright. Wright's paint is prepared from eosine (see) and methylene blue (see) as follows: 1% methylene blue in 0,5% water solution of baking soda pour solution of the basic in a wide flask so that thickness of a paint layer did not exceed 6 cm; heat in Koch's device or on the water bath at t ° 100 ° within an hour from the moment of formation of vapors. At artificial lighting the cooled filtrate of paint in a thin coat shall have the purple-red shade caused by formation of an azur. Add 500 ml of 0,1% of water solution of eosine to 100 ml of a filtrate of paint. At the same time the plentiful deposit is formed. Mix is shaken up and quickly filtered. The paper filter is dried up together with a deposit then powder is collected and the received Wright's paint is dissolved in methyl alcohol (0,1 g of paint on 60 ml of alcohol).

Way of coloring: on a dry blood smear for its fixing (see) pour several drops of not divorced paint of Wright and maintain 1 min., then add the same quantity of drops of a distilled water and maintain 3 min. Paint is washed away water, drug is dried filter paper. Azur who is in paint paints kernels of cells in cerise color and reveals azurophilic granularity in leukocytes, lymphocytes, is especially accurate — in monocytes.

See also Histologic methods of a research .


Bibliography: Wright J. N of A rapid method for the differential staining of blood films and malarial parasites, J. med. Res., v. 7, p. 138, 1902.

S. V. Prozorovsky.

Яндекс.Метрика