UNNY — the TENDER the METHOD (
P. G. Unna, is mute. dermatologist, 1850 — 1929; P. Taenzer, is mute. the dermatologist, 1858 — 1919) — a way of coloring of elastic fibers of connecting fabric spirit of orcein. The method is offered by Tentser, modified and entered into practice by P. Unna (1891).
Material is fixed, washed out and if necessary prepare cuts; paraffin sections dewax. After washing cuts or whole mounts immerse on 1 — 18 hour at the room temperature or for 10 — 30 min. at t ° 30 — 37 ° in fresh 1% solution of orcein (see) in 65 — 100% alcohol, 1 ml salt to - you is added to 100 ml to-rogo. If necessary coloring is differentiated alcohol, drugs dehydrate and conclude in balm. Elastic fibers gain brown-red color; if after a differentiation to process drug vapors of ammonia, coloring darkens and becomes bluish. Efficiency of coloring in many respects depends on quality of orcein; at high quality of dye of fiber are accurately visible, up to their thinnest branchings.
See also Histologic methods of a research.
Bibliography: L and l of l and R. Patogistologicheskaya of the technician and a practical histochemistry, the lane with English, page 510, M., 1969; M e republics at l about in G. A. Kurs of the patologogistologichesky equipment, page 191, L., 1969;
Unna P. Notiz betreffend die Tanzer’sche Orceinfarbung des elastischen Gewebes, Mh. prakt. Derm., Bd 12, S. 394, 1891. I. E. Hesinonim