SUDAN

From Big Medical Encyclopedia

SUDAN (Sudan) — the name of group of the indifferent dyes applied in histologic researches to identification of lipids.

Dyes of group C. belong to substantive to dyes (see). They are well dissolved in fats thanks to what lipidosoderzhashchy structures of fabrics remove S. from the painting solution, is slightly weaker — in alcohols, acetone; are water-insoluble.

By the chemical nature different types of S. are heterogeneous. Carry Sudan to monoazoic dyes of II, Sudan brown, Sudan red In, Sudan Corinthian STARS; to bis azoic dyes — Sudan III, Sudan IV (sharla red), Sudan red 4BA (fatty red 4B), Sudan red VIIB (fatty red VIIB), Sudan black In; to aminoanthraquinone — Sudan violet, Sudan blue, Sudan green. For coloring of drugs C. (mix C. of one chemical group is better) dissolve in 40 — 70% alcohol, mixes of alcohol with acetone, 50% diacetin, propylene glycol or other organic solvents before saturation and paint of 15 min. to 18 — 24 hours; lipidosoderzhashchy structures gain the corresponding S.'s color (see. Lipids ). After washing drugs are concluded in polyvinyl alcohol, glycerin-gelatin or gum-syrup. Apply Sudan III, Sudan IV and Sudan black more often In; they are suitable not only for coloring gistol. drugs, but also for studying of lipoproteids by method of paper electrophoresis (Sudan III and Sudan IV), in agarose or on acetate-cellulose (Sudan black In and Sudan red 4BA).

See also Histologic methods of a research , Substantive coloring .


Bibliography: Lille R. Patogistologicheskaya of the technician and a practical histochemistry, the lane with English, page 122, 421, M., 1969; Merkulov G. A. Course of the patologogistologichesky equipment, page 240, L., 1969.


Ya. E. Hesinonim

Яндекс.Метрика