SHPALTEGOLTSA METHOD

From Big Medical Encyclopedia

SHPALTEGOLTSA MYOTOD (W. Spal-teholz, is mute. the anatomist, 1861 — 1940) — a way of preparation of the transparent or brightened-up anatomic drugs.

The method is developed by V. Shpaltegolts in 1911. The Shpalte-goltsa method is the basic for production of the brightened-up anatomic drugs, to-rye give the chance against the background of transparent fabric to study intraorganic vessels, nerves, ossification centers (see Drugs anatomic).


The essence of a method is that the opaque object which is subject to a research is impregnated with substance, the index of refraction of light in Krom matches or is close to that in fabrics of an object therefore fabrics become transparent.

After pre-treatment (an injection of vessels, coloring of a bone tissue, cartilage, nerves, etc.) drugs fix in 5 — 10% solution of formalin, decalcify (if a subject of studying are bones), bleach 3 — 5% solution of hydrogen peroxide (if drugs are not painted), wash out in a distilled water and dehydrate in alcohols of the ascending fortress from 50% to absolute, then under an air chamber delete air traps and place in the clarifying liquid.

As the clarifying liquids Shpaltegolts at first used mix of benzene with carbon sulfur, and further began to apply more indifferent substances — methyl ether salicylic to - you, benzyl benzoate, gaulterovy oil, isosafrol usually in a combination with each other in various ratios. Ready drugs hermetically close in banks by means of the putty consisting of 50 g of arabic gum, 50 g of sugar, 2 g of soluble glass and 1 g of formalin.

Bibliography: Residents of Yaroslavl

B. M. Anatomicheskaya of the technician, page 228, Frunze, 1961; Spalteholz W. t)ber das Durchsichtigmachen von menschlichen und tierischen Praparaten; nebst Anhang, Uber Knochenfarbung, Lpz., 1911.

V. S. Speransky.

Яндекс.Метрика