From Big Medical Encyclopedia

RAMON-I-CAJALYA METHODS (S. Ramon at Cajal, the Spanish histologist, 1852 — 1934) — the histologic methods of identification of structures of nervous tissue by processing of drugs salts of heavy metals (impregnation) offered S. Ramone-and-Kakhalem. Among these methods distinguish the modification of a method of Golgi directed to identification of neurocytes, a method of identification of neurofibrilla and methods of impregnation of a neuroglia.

Modification of a method of Golgi, is developed in 1888. Processed by Golgi's method (see. Golgi method ) pieces of fabric immerse in the liquids which were already in the use again — at first in the solution containing potassium bichromate and osmic to - that, then in solution of caustic silver.

The method of identification of neurofibrilla offered in 1903 is based on silver recovery in impregnirovaniy nervous tissue. S. Ramone-and-Kakhalem several modifications of this method in relation to different objects are offered. For identification of neurocytes of a cerebral cortex and granular neurocytes (cells grains) of a cerebellum fresh pieces of fabric maintain in 0,75 — 3% solution of caustic silver at 1 ° 35 ° within 3 — 5 days before emergence of tobacco-brown coloring, after that wash out them in a distilled water within 1 min. and postpone to 24 hours in the liquid containing 1 — 2 g pyrogallic to - you or hydrochinone, 5 ml of neutral formalin and 100 ml of a distilled water, wash out again and conclude in photoxylin or paraffin.

Methods of impregnation of a neuroglia

1. Impregnation by gold with corrosive sublimate is offered in 1926. Pieces of fabric fix in bromine-formole (15 ml of neutral formalin, 85 ml of a distilled water and 2 g of ammonium bromide) within 2 — 20 days, frozen sections transfer to the fixing liquid, rinse in water and place on 4 — 8 hours at 7 ° 18 — 22 ° in the freshly cooked solution containing 0,5 — 0,8 g of corrosive sublimate in 50 ml of a distilled water. Add 6 ml of 1% of solution of chemically pure chloric gold to this solution. Then cuts well wash out and fix 6 — 10 min. in 5% solution of sodium hyposulphite or in the fixer Ramón-and-Kakhalya (70 ml of 5% of solution of sodium hyposulphite, 30 ml of 96% of alcohol and 5 ml of the concentrated solution of sodium bisulphite), wash in 50% alcohol, transfer to a slide plate, dry filter paper, apply absolute alcohol, then cretian origanum oil, a xylol, balm. Cells of a neuroglia are painted in purple-red color.

2. Impregnation by silver about bromine - formoly, offered in 1924, is applied to studying of gliotsit and their shoots. This method allows to reveal also basphilic substance (Nissl's little body) in neurocytes. Pieces of fabric fix bromine-formolom as in the previous method, frozen sections place in the amplifier (70 ml of a distilled water, 30 ml of neutral formalin and 3 g of ammonium bromide), wash out a distilled water and immerse for 5 — 10 min. in the mix containing 10 ml of ammoniac silver, 10 — 12 ml of a distilled water and 7 — 10 drops of pyridine. Then mix is warmed up before acquisition by cuts of tobacco coloring, washed out 2 times in a distilled water, recovered in 5% neutral formalin within 2 — 3 min., gilded in 0,2% solution of chloric gold, fixed in 5% solution of sodium hyposulphite, washed out, dried, dehydrated alcohol and a xylol and concluded in balm.

3. The method of impregnation by uranium with formoly, offered in 1912, is used for coloring of granules of a protein and drops of fat in gliotsita. The same method with the shortened time of fixing is applied to identification of a complex of Golgi.

See also Histologic methods of a research , Gilding methods , Impregnation , Silverings methods .

Bibliography: Romeys B. Mikroskopicheskaya of the technician, the lane with it., M., 1954; R and-tbp at With a j and 1 S. Sobre un sencillo procéder de impregnaciön de las fibrillas interiores del protoplasma nervioso, Arch, lat. med. biol., Madrid, v. 1, p. «3, 1903; it, Elementos de técnica microgrâfica de] sistema nervioso, Madrid, 1933.

V. S. Speransky.