PYRONIN

From Big Medical Encyclopedia

PYRONIN — the main red dye of group of xanthenes applied in histologic methods of a research to coloring of kernels of cells, basphilic substance of cytoplasm, peroksidazny granules of leukocytes, and also bacteria, etc. Solutions P. fluoresce. Distinguish P. of brand Y, or G (yellowish) representing tetrametildiaminoksantenil-chloride, and P. of brand B (bluish) differing in existence of ethyl groups instead of methyl. II. we will well dissolve brands B in water and alcohol; P.'s crystals of the Y(G) brand of a rastvorima in water (to 8,96 g on 100 ml) and in alcohol (to 0,6 g on 100 ml). Y(G) pyronin has elective affinity to ribonucleic to - there cells in this connection in mix with imperial green it is applied in a histochemistry to identification nucleinic to - t (see. RNA , Unna — Pappengeyma a method ). Karnik (N. Century of Kurnick, 1955) and some other researchers consider that only P. of the Y(G) brand since pyronin B gives only nonspecific coloring of cytoplasmatic components, but Pearce (A is suitable for this purpose. G. E. Pearse, 1962) recommends P. of brand B. Before the use dye of both specified brands quite often should be purified of impurity washing in chloroform.



Bibliography: Lille R. Patogistologicheskaya of the technician and a practical histochemistry, the lane with English, page 112, etc., M., 1969; Pearce E. A histochemistry, the lane with English, page 188, etc., M., 1962; Kurnick N. Century of Pyronin Y in the methyl-green-pyronin histological stain, Stain Technol., v. 30, p. 213, 1955.


I. E. Hesinonim

Яндекс.Метрика