PSHENICHNOVA METHOD (A. V. Pshenichnov, owls. the microbiologist, 1900 — 1975) — the method used for infection of the monoksenny parasites eating only blood of the person.
It is offered in 1942 by A. V. Pshenichnov. The method allows to infect insects in the conditions as close as possible to natural. It can be used for studying of development rickettsiae (see) in an organism of a carrier, and also resistance of rickettsiae to disinsection agents. Items of m apply in epidemiol. to practice for allocation from blood of causative agents of a typhus.
Between two disks of a special feeding trough pull a membrane. As the best membrane serves the thinnest layer of epidermis (epidermomembran) of skin of a corpse subjected to easy heat treatment. Under a membrane the syringe bring the defibrinated blood of the person containing any microorganisms. Insects stick to a membrane, suck blood and thus are infected.
The main advantages of a method — a possibility of dosing of microorganisms and infection of a large number of insects in a short space of time.
Bibliography: Kostromina E. E. K to a question of the nature of postinfectious immunity at an experimental sapropyra, in book: Materials konf. on rik-kets. and virusn. infekts. person, page 22, Perm, 1957; Pshenichnov A. V. Experience of fifteen years' use of a method epidermomembran (method of infection of blood-sicking insects), Works Permsk. nauch. - issled. in-that vaccines and serums, century 1, page 84, 1959; it, the Universal method of studying of the infections transmitted to the person by blood-sicking insects and a new vaccine against a typhus, Zhurn. mikr., epid. and immun., JvTs 1-2, page 43, 1943; Pshenichnov A. V., Shevelyova O. N. and Noskova E. G. Variability of rickettsiae of Pro-vacheka and perspective of receiving a live typhous vaccine, in the same place, No. 7, page 11, 1957.
A. V. Pshenichnov.