PLAZMALEVY REACTION

From Big Medical Encyclopedia

PLAZMALEVY REACTION — the histochemical reaction allowing to reveal eurysynusic in tissues of animals and the person of an atsetalfosfolipida, being in connection with phosphatides. It is offered in 1924 by R. Feulgen and K. Foyt. Researchers called substances of the lipidic nature in which in acid medium aldehydes, by plasmogenes, and aldehydes — plasmals are formed; from here name of the reaction. Has no independent value P. of river, however it is important for the correct interpretation of other histochemical reactions which are carried out by means of Schiff's reactant, napr at identification of DNA, neutral glikozaminoglikan, etc. P. of river can be used for confirmation of existence in fabrics of an atsetalfosfolipid, ethanolamine, the cholinecontaining phospholipids if absence in these substances of neutral fats is proved. In the course of P. the river bares aldehydic groups of atse-talfosfolipid and these connections give with Schiff's reactant (see. Schiff reactant ) reddish-purple coloring. True P. of river at prolongation of fixing of material gradually weakens; it is strengthened by corrosive sublimate, releasing aldehydes of the highest fat to - t from atsetalfosfo-lipids. In certain cases there can be a psevdoplazmalevy reaction which depends on presence at fabrics of the oxidized phosphatides; corrosive sublimate does not exert impact on it and increase in terms of fixing of material strengthens reaction.

Items of river on Heysa (E. R. Hayes, 1949) carry out on unstable smears or cuts 10 — 15 microns thick at made on the freezing microtome or in the cryostat. Cuts wash out in a distilled water, one cut place in 1% water solution of corrosive sublimate for 2 — 10 min., then this cut and (control) which is not processed by corrosive sublimate paint Schiff's reactant within 5 — 15 min. Further both cuts wash out in three portions of bisulfite water (5 ml of 10% of K 2 S 2 O 5 , 5 ml 1 N of HCl solution, 100 ml of a distilled water], 2 min. in every portion, then in water, carry out through alcohols and a xylol and conclude in glycerin-gelatin. As a result of reaction of an atsetalfosfolipida are painted in reddish purple - ny color; in control reaction shall be negative.

Items of river according to Kane (J. A. Cain, 1949) carry out in a piece of unstable fabric which is placed for 15 min. in mix of equal parts of a reactant of Schiff half divorced bisulfite water and sated (7%) of water solution of corrosive sublimate. Then the piece is washed out in several portions of bisulfite water, fixed 2 hours in 10% formalin, washed out 2 hours in running water and from it prepare the frozen or cryostately cuts, to-rye later dehydration in alcohols and enlightenments in a xylol concluded in glycerin-gelatin. As a result of reaction of an atsetalfosfolipida are painted in a reddish magenta color.



Bibliography: Lille R. D. Patogistologicheskaya of the technician and a practical histochemistry, the lane with English, M., 1969; Jlynna X. Bases of a histochemistry, the lane with it., page 96, 171, M., 1980; Pearce E. A histochemistry, the lane with English, M., 1962; P about with to and G. I. N and JI e-vinson JI. B. Mikroskopicheskaya of the technician, L., 1957; F e u 1 g e n R. u. Y about i t K. t)ber einen weitverbreitenten festen Aldeh id „Pfltigers Arch. ges. Physiol., Bd 206, S. 389, 1924.


N of T. Raykhlin.

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