PIKVORTA METHOD

From Big Medical Encyclopedia

PIKVORTA METHOD (F. A. Pickworth, the English anatomist of 20 century) — a way of identification of blood vessels, including also capillaries, by processing of cuts of fabrics mix of benzidine with Sodium nitroprussidum. It is offered in 1934. The method is based on peroksidazny activity of hemoglobin, i.e. on its ability to catalyze redoxreaction in which as electron sink serves hydrogen peroxide (see. Peroxidases ). Benzidine during the processing of drugs according to P. of m is oxidized, forming unstable the benzidine blue in the beginning, and then steadier connection of brown-black color. On Doherty's modification, Sakai Alexander (M. M. of Doherty, T. H. Suk, L. Alexander), from the material fixed by formalin frozen sections prepare thick (200 — 300 microns), to-rye later washing in a distilled water within 10 min. process freshly cooked mix 50 ml of 1% of solution of benzidine in absolute alcohol and 10 ml of 1% of water solution of Sodium nitroprussidum; mix is added a distilled water to 100 ml. After short-term washing oxidation is completed in the mix consisting of absolute alcohol (50 ml) ice acetic to - you (2 ml), pergidrolya (0,5 ml), Sodium nitroprussidum (0,1 g) and a distilled water (to 100 ml). After washing drugs dehydrate in acidified acetic to - by that (to 2%) alcohols of the ascending fortress, carry out through absolute alcohol and a xylol, conclude in balm; store in the dark. On the drugs processed according to P. the m filled with erythrocytes blood vessels are painted in black color and are visible on a light background.

See also Histologic methods of a research .



Bibliography: Romeys B. Mikroskopicheskaya of the technician, the lane with it., page 484, M., 1954; P i with k w about r t h F. And. A new method of study of the brain capillaries and Its application to the regional localization ol mental disorder, J. Anat. (Lond.), v. 69, p. 62, 1934, bibliogr.


I. E. Hesinonim

Яндекс.Метрика