PERLSA WAY

From Big Medical Encyclopedia

PERLSA WAY (The m of Perls, is mute. the pathologist, 1843 — 1881) — a method of identification in microscopic sections of salts of iron oxide. The item of page is offered in 1867. It is applied to identification of salts of iron oxide in cells and fabrics at disturbances of pigmental exchange, napr, at hemosiderosis (see), and also for the purpose of differentiation of hemosiderin, in particular with melanin, for determination of prescription of hemorrhages, etc. For partial unmasking of the connected iron cuts process on Kokkel's way (see. Kokkelya ways ) or way microincinerations (see).

Pieces of fabric fix in 10% formalin or in Bowen's mix. Gistol, cuts are prepared after freezing of pieces of fabric or their filling in paraffin (see. Histologic methods of a research ). The cuts which are washed out by a distilled water for 30 — 60 min. transfer to the mix consisting of the equal volume of 2% of solution salt to - you and 2% of solution geksatsiano-(P) ferrate of potassium (yellow blood salt). Then wash out in a distilled water (5 — 10 min.), dokrashivat kernels of 1% water solution of neutral red or an aluminous carmine (3 — 5 min.), rinse, dehydrate and conclude in balm or synthetic medium. Water and salt to - that, the pages used at P., shall not contain ions of iron, to-rye can pervert result of reaction. During the processing gistol, cuts it is necessary to use glass microscopic needles.

As a result of P. the pages of structure containing salts of iron oxide are painted in dark blue color, and kernels of cells — in red.


Bibliography: Lille R. Patogistologicheskaya of the technician and a practical histochemistry, the lane with English, page 376, M., 1969; Magnifying glasses pases of X. Bases of a histochemistry, the lane with it., M., 1980; Merkulov G. A. Course of the patogistologichesky equipment, page 283, L., 1969; Peris M. Lehrbuch der allgemeinen Pathologie fiir Studierende und Aerzte, Stuttgart, 1877 — 1879.


G. M. Mogilevsky.

Яндекс.Метрика