NORIMBERSKY METHOD (J. K. Norymberski) — a chemical method of quantitative definition of corticosteroids and their metabolites (17-ketogenic steroids) in urine; in a wedge, practice it is applied at diagnosis of disturbances of glucocorticoid function of bark of adrenal glands. The N of m is based on property of corticosteroids at their soft oxidation vismutaty sodium (NaBiO 3 ) to form 17 ketosteroids (see).
For the first time the method was offered
N., No-rimbersky in 1952, by m based on oxidation — C20 — C21 of a side chain of a steroid kernel characteristic of corticosteroids therefore 17 connections of ketosteroids which are quantitatively defined by means of staining reaction of Tsimmermann with m dinitrobenzene are formed. The initial version of N. of m consists of the following main stages: 1) determination of initial content of 17 ketosteroids (metabolites of androgens) in urine by means of Tsimmermann's reaction; 2) oxidation of steroids of urine vismutaty sodium; 3) repeated determination of content of 17 ketosteroids, a cut now represents the sum of concentration of initial 17 ketosteroids (metabolites of androgens) and 17 ketosteroids formed as a result of oxidation of corticosteroids and their metabolites (so-called ketogenic steroids). Concentration of ketogenic steroids is determined by a difference between the maintenance of 17 ketosteroids after oxidation vismutaty sodium and initial concentration of 17 ketosteroids in the studied sample of urine. The concentration of ketogenic steroids determined by N. in m in its such option represents the sum of concentration of cortisol, cortisone, 11 - zoksikortizola and their main metabolites — tetrahydroderivatives, cortols, kortolon, and also a pre-gnantriol.
At men excretion of ketogenic steroids with urine normal makes 12,83 +-0,8 mg a day, at women — 10,61 +-0,66 mg a day.
Later a number of modifications of N. of m with the purpose to make this method more convenient for performance, to increase reproducibility of results, and also to expand a range of the steroids defined with its help was offered. N.'s modifications m consist hl. obr. in change of conditions of oxidation of steroids of urine. Modification of Applbi — Norimbersky is most widespread, according to a cut oxidation of steroids vismutaty sodium is made after pretreatment of the studied urine borgidridy sodium. In these conditions are also exposed to oxidation to 17 ketosteroids 17-oksipregnenolon and 17 pregnenoldione, and initial 17 ketosteroids are recovered to 17 hydroxysteroids and are not defined. During the use of this modification of N. of m single definition of 17 ketosteroids after recovery borgidridy and oxidations vismutaty sodium allows to measure the summary contents of ketogenic steroids, in a cut enter in addition in comparison with initial option H. of m 17-oksipregnenolon and 17 pregnenoldione.
Between the speed of secretion of cortisol and excretion of 17-ketogenic steroids there is directly proportional dependence, at the same time daily excretion of 17-ketogenic steroids makes about a half of the cortisol cosecreted by adrenal glands.
See also Corticosteroids .
Bibliography: Yudayev N. A. Chemical methods of definition of steroid hormones in biological liquids, M., 1961; Appleby J. I. and. lake of Indirect analysis of corticosteroids, Biochem. J., v. 60, p. 453, 1955; Brooks G. J. W. a. Norymberski J. K. The oxidation of corticosteroids with sodium bismuthate, ibid., v. 55, p. 371, 1953; Hormones in blood, ed. by G. H. Gray a. Y. H. T. James, v. 3, p. 180, L. a. o., 1979.
V. S. Zelenetskaya.