From Big Medical Encyclopedia

NOBLYA REACTION (A. Noble) — the accelerated approximate method of agglutination of bacteria applied to detection of antibodies in blood serum of the patient. It is offered by Nobl in 1927. The hl is used. obr. as an approximate method at diagnosis of rickettsioses, a brucellosis and nek-ry others inf. diseases.

The received results at N. of river, as a rule, match data of other agglutination tests, but N. the river does not allow to define precisely an antiserum capacity in blood serum of the patient.

Blood serum of the patient gets divorced isotonic solution of sodium chloride 1:10, 1:20, 1:40, 1:80. Antigen are the dense microbic suspensions containing 3 — 5 billion microbic bodies in 1 ml (see. Diagnosticums ). As antigen can be used as well diagnosticums, the preserved 0,2% by solution of formalin. At N.'s statement by the river in agglyutinatsionny test tubes measure 0,1 ml of the corresponding cultivations of blood serum and 0,1 ml of the concentrated antigen. The control test tube contains 0,1 ml of isotonic solution of sodium chloride and 0,1 ml of antigen. The support with test tubes is vigorously stirred up within 2 — 5 min. Then, including control, bring 0,8 ml of isotonic solution of sodium chloride in all test tubes. Addition of isotonic solution of sodium chloride does not change cultivation of blood serum since it is made after completion of process of pasting of antibodies with antigens. Reaction is considered by means of an agglyutinoskop.

N.'s advantage of river is that for statement of reaction use the small volume of blood serum of the patient, quickly receive result, there is no need of use of the thermostat.

See also Agglutination .

Bibliography: The guide to microbiological diagnosis of infectious diseases, under the editorship of K. I. Matveev, page 56, M., 1973; The Reference book on microbiological and virologic methods of a research, under the editorship of M. O. Birger, page 310, M., 1973; Noble A. A rapid method for the macroscopic agglutination test, J. Bact., Y. 14, p. 287, 1927.

S. A. Efremova.