NILANDERA TEST

From Big Medical Encyclopedia

NILANDERA TEST (Page W. G. Nylander, the Swedish chemist, 1835 — 1907) — qualitative test on presence of glucose at urine. It is offered in 1883. It is used in need of the emergency definition of glucose in urine at a diabetes mellitus, poisonings with carbon monoxide, hydrocianic to - that, by mercury, at inf. diseases, etc.

N. of the item, as well as some other qualitative tests on glucose (e.g., test of Felinga), is based on the reducing (recovering) properties of aldehydic group of glucose. As an oxidizer use any easily recovered connection giving the painted reaction product at recovery. In test of Felinga such connection is Cu(OH) 2 , and in N. of the item — bismuth hydroxide — BiOH) 3 , edges it is recovered by glucose to BiO of brown color and metal bismuth of black color. The N of the item is carried out in alkaline condition, in a cut glucose from a cyclic form passes into a form with not closed circuit and free carbonyl group [[ File: | | alt= glucose from a cyclic form passes Nylander.png with x40px into a form with not closed circuit and free carbonyl group | glucose from a cyclic form passes into a form with not closed circuit and free carbonyl group ]] , causing the recovering properties of glucose. Besides, in alkaline condition the return oxidation of the painted reaction product since it drops out in a deposit is prevented and comes out reaction.

Nilander's reactant contains seignette salt, about a cut of Bi (NO 3 ) 3 forms the alcoholate of bismuth which is hydrolyzed with release of bismuth hydroxide, the quantity a cut corresponds to amount of the defined glucose.

Course of definition. Several milliliters of urine mix with Nilander's reactant in the ratio 3:1: mix is heated within 3 — 4 min. on a flame. At positive H. items observe emergence of coloring from brown to black and opacification. At negative test the colourless deposit of phosphates drops out.

N.'s sensitivity of the item — 0,1% of glucose. Foreign substances (nek-ry pharmaceuticals, uric to - that, etc.) exert impact on N. of the item only if they are in urine in considerable concentration.


Bibliography: Biochemical methods of a research in clinic, under the editorship of A. A. Pokrovsky, page 266, M., 1969; Nylander E. Uber alkalische Wismuthlosung als Rea-gens auf Traubenzucker im Harne, Z. physiol. Chem., Bd 8, p. 175, 1883—1884.


H. G. Budkovskaya.

Яндекс.Метрика