MIDDLBRUKA-DYUBO REACTION (G. Middlebrook, sovr, American bacteriologist; R. J. Dubos, the French biochemist) — the serological test consisting in agglutination of the erythrocytes loaded with the antigen of mycobacteria of tuberculosis, serums containing the corresponding antibodies. It is offered in 1948 for definition of the agglutinating antibodies against antigens of the causative agent of tuberculosis. M — D. of river — option of reaction of indirect hemagglutination. In 1950 Middlbrukom is developed modification of reaction, at a cut the result is considered on the hemolysis of the loaded erythrocytes caused by addition complement (see).
M — D. the river is founded on ability of native erythrocytes to occlude nonprotein antigens on a surface and to be agglutinated by antibodies to these antigens. Erythrocytes are the carrier of antigen and the indicator of reaction. Reaction is used as auxiliary diagnostic test at tuberculosis. Owing to an antigenic affinity of causative agents of tuberculosis and a leprosy reaction was used also for diagnosis of the last.
Subject to a research blood serum of the inspected persons. The procedure of a research includes preparation of the loaded erythrocytes and preliminary exhaustion of the studied serums native erythrocytes in order to avoid nonspecific reactions. As the carrier 0(1) blood groups or erythrocytes of a ram use erythrocytes of the person. Tuberculine, polisakharidny or lipopolisakharidny extract of mycobacteria can be antigens. The washed erythrocytes mix with antigen, maintain 2 hours at t ° 37 °, wash by means of the centrifuge from excess of antigen, prepare 0,5% a suspension. Statement and the accounting of reaction — as well as at passive hemagglutination and Boyden's reaction (see. Boydena reaction ). Reaction is regarded as positive if agglutination is caused by ispytuyemy serum, divorced 1: 8 and more, in the absence of agglutination by serum of the control not loaded erythrocytes. Positive reaction is caused by 60 — 90% of blood sera of TB patients and 1 — 2% of blood sera of almost healthy people.
Bibliography: Theologian of E. M. Clinical value of hemagglutination reaction at an active pulmonary tuberculosis, Zdravookhr. Belarus, No. 4, page 38, 1959; Middlebrook G. Hemolytic modification of hemagglutination test for antibodies against tubercle bacillus antigens, J. clin. Invest., v. 29, p. 1480, 1950; Middlebrook G. Dubos R. J. Specific serum agglutination of erythrocytes sensitized with extracts of tubercle bacilli, J. exp. Med., v. 88, p. 521, 1948; S t r u at H. Der Einsatz gereinigter Lipopo-lysaccharide zur serologischen Diagnostik der Tuberkulose, Z. ges. Hyg., Bd 23, S. 390, 1977.
B. H. Sofronov.