LIBERMANNA-BURHARDA REACTION (L. Liebermann, Wenger, biochemist, 1852 — 1926; The N of Burchard) — qualitative staining reaction on cholesterol, at interaction to-rogo with mix the ice acetic and concentrated chamois of acids and acetic anhydride develops emerald-green coloring. L. — The B. river is the cornerstone of the majority of methods of definition of the general cholesterol and its fractions in blood serum which are widely applied in a wedge, practice, in particular the unified method of definition of cholesterol by a method of the Fisher (see. Fishers method ).
Reaction is opened in 1885 for Libermannom and applied to definition of cholesterol in blood serum of Borax hard in 1890.
The methods based on L. — B. rubles, have good reproducibility. A lack of reaction is its light sensitivity, moisture and to changes of temperature, besides, of L. — The B. river on is absolutely specific to cholesterol: holestanol (degidrokholesterin), Δ-7-holestanol, 7-degidrokholesterin and others sterols (see) give the same coloring with mix the ice acetic and concentrated chamois to - t and acetic anhydride, as cholesterol.
See also Holesterin .
Bibliography: Rosenzweig K. I. The accelerated method of definition of the general cholesterol on the Fisher, Laborat, business, No. 9, page 43, 1962; Bure hard H. Beitrage zur Kenntnis des Chc1osterins, Chem. Zbl., Bd 1, S. 25, 1890; Liebermann L. t)ber das Oxychinoterpen, Ber. dtsch. chem. Ges., S. 1803, 1885.
L. H. Delektorskaya.