LEPENE WAY (G. Lepehne, is mute. doctor, sort. in 1887) — a histologic way of identification intracellular and extracellular hemoglobin .
Hp is applied to detection in gistol, drugs of circulatory capillaries, small hemorrhages, etc. The method is based on detection of peroksidazny properties of hemoglobin, to-rye depend on catalytic properties of the iron which is contained in it. It is offered in 1919.
Fabric is fixed in formalin. The material which is long stored in formalin is unsuitable. The frozen or paraffin sections transfer from water and place for 1 — 5 min. in freshly cooked solution of benzidine with hydrogen peroxide (2 ml of 0,6% of solution of benzidine in 96% alcohol, 0,5 ml of hydrogen peroxide, 4,5 ml of 70% of alcohol). Then cuts wash out at first in 50% alcohol, and then quickly in a distilled water; if necessary cuts paint a carmine or aluminous hematoxylin for identification of kernels of cells. Cuts dehydrate and conclude in Canada balsam: erythrocytes and free hemoglobin are painted in dark brown color.
For the differentiated identification of hemoglobin and hemosiderin cuts before coloring of cellular kernels place in mix 2% of solution of ferroprussiate and 1% of solution salt to - you, taken in equal quantities; at the same time hemoglobin remains dark brown, and hemosiderin is painted in blue color. Unstable smears place in a reactant for 10 min. (instead of 70% take 96% alcohol) then they are washed out and process also; coloring remains not for long.
Bibliography: Romeys B. Mikroskopicheskaya of the technician, the lane with it., page 339, M., 1954; Lepehne G. Zerfall der roten Blutkorperchen beirn Ikterus infectiosus (Weil), Beitr. path. Anat., Bd 65, S. 163, 1919, Bibliogr.
I. E. Hesinonim