KVIKA-PYTELYA TEST (A. J. Quick, amer. doctor, sort. in 1894; A. Ya. Pytel , Soviet urologist, sort. in 1902) — a method of a research of anti-toxic function of a liver by determination of amount of hippuric acid in urine after introduction to an organism of the sodium benzoate.
It is offered by Kvik in 1933, for a wedge, uses it is modified by A. Ya. Pytel in 1941.
Destruction of the sodium benzoate happens in a liver at connection of the benzoic to - you to glycine in the presence of enzyme of a gippuraza; as a result of it it is formed benzaminoacetic to - that, allocated with urine. The reduced education in a liver benzaminoacetic to - you can depend on insufficient ability of hepatic cells to synthesize glycine, on damage of the enzymatic mechanism controlling connection of the benzoic to - you with glycine. Exist oral and intravenous To. — Pct
Hour later later breakfast consisting of a glass of coffee and 50 g of crackers, give to the patient in 4 g of the sodium benzoate dissolved in 30 ml of water. The accepted sodium benzoate of the patient washes down with a glass of water and right there empties a bladder for receiving control urine. During 4 hours hourly collect urine and in every portion determine content benzaminoacetic to - you. Each of the received 4 portions of urine and a portion of control urine are acidified by 1 — 2 ml konts. salt to - you before emergence of a reddish shade also investigate an indicator piece of paper with congo red; if the piece of paper becomes blue, add some more drops to - you. Every portion urine carefully shake up until sedimentation of crystals benzaminoacetic to - you are full. Then the deposit is left to stand within an hour at the room temperature. Benzaminoacetic to - that is filtered on in advance tared filters, washed out a distilled water and dried up to constant weight (in a drying oven or on a suspended framework over an electric tile). Quantity benzaminoacetic to - you determine by either weighing, or titration by two-decinormal solution of caustic soda with phenolphthalein. At calculation to the received quantity benzaminoacetic to - you add the quantity it which is usually contained always in urine in the dissolved state (100 ml at the room temperature contain 0,33 g benzaminoacetic to - you). Subtract the weight of the filter and weight from the received figure benzaminoacetic to - you in the control urine collected before conducting this test. The received number is multiplied by coefficient 0,68 benzaminoacetic to - you to express quantity in equivalent amounts of the sodium benzoate.
Intravenous test is carried out at disturbances of secretory and motor function went. - kish. path. To the patient several minutes prior to administration of solution of the sodium benzoate allow to drink 2/z a glass of rather weak tea or coffee. Then the patient empties a bladder for receiving control urine. After that intravenously slowly enter freshly cooked solution of the sodium benzoate (1,77 g of the sodium benzoate dissolved in 20 ml of a distilled water). Hour and two hours of the patient later urinates again. To each 10 ml of collected urine add 5 g of solid ammonium sulfate and determine quantity benzaminoacetic to - you by a technique of oral test.
Most of healthy people distinguishes within the first hour 0,7 — 0,95 g benzaminoacetic to - you. Its definition in two portions is caused by the fact that normal quantity benzaminoacetic to - you can be allocated within not the first, but the second hour of a research.
Results of definition are expressed percentage of that quantity benzaminoacetic to - you, a cut is allocated normal.
About disturbance of anti-toxic function liver (see) it is possible to speak if less than 80% of the sodium benzoate accepted inside are allocated (not less than 3 g benzaminoacetic to - you). The sharp deviation in fractional allocation benzaminoacetic to - you at its reduced total quantity is considered as display of the latent liver failure and is observed at various acute and hron, parenchymatous damages of a liver; at mechanical jaundice test does not give aberrations.
Reduction of allocation benzaminoacetic to - you are observed at diseases went. - kish. a path, insufficiency of protein in food, frustration funkts, conditions of kidneys, at pregnancy, anemias, a diabetes mellitus, malignant new growths. For the correct assessment of results To. — The pct should exclude these states.
Bibliography: Cooper 3. A. Clinical hepathology, page 75, M., 1970; Pytel A. Ya. A functional trial of a liver of Kvik in clinic of surgical diseases, Klin, medical, t. 23, No. 4-5, page 42, 1945; Shelagurova. A. Methods of a research in clinic of internal diseases, page 260, M., 1960; Q u i with k A. J. Synthesis of hippuric acid, Amer. J. med. Sci., v. 185, p. 630, 1933; Quick A. J., OttensteinH. N.a. WeltchekH. Synthesis of hippuric acid in man following intravenous injection of sodium benzoate, Proc. Soc. exp. Biol. (N. Y.), v. 38, p. 77, 1938.
A. Ya. Pytel.