KULCHITSKY METHOD

From Big Medical Encyclopedia

KULCHITSKY METHOD (N. K. Kulchitsky, the domestic histologist, 1856 — 1925) — a way of coloring of myelin covers of nerve fibrils. Is one of the best modifications of a method of Veygert (see. Veygerta methods of coloring ). The method is offered in 1890. Pieces fix in 10% solution of formalin or in Müller's liquid (in the first case they are in addition chromeplated) and fill in in photoxylin. Cuts paint 12 — 24 hours in mixes: ripened (sustained within not less than 7 days) 10% solution of hematoxylin in absolute alcohol — 10 ml, a distilled water — 90 ml, ice acetic to - they are 2 ml. Differentiate 4 — 12 hours in mix: the concentrated water solution of lithium carbonate — 100 ml, 1% water solution of red prussiate of potash of 10 ml (liquid is replaced 1 — 2 time). Then cuts wash out in running water, dehydrate and conclude in Canada balsam. Myelin nerve fibrils are painted in black color. If coloring is insufficiently elective, it is possible to chromeplate cuts in addition (8 — 14 days) in Müller's liquid or to ship for days in 1% solution chromic to - you, to wash out a dist, water and to paint repeatedly. Voltaire (M. of Wolters, 1890) recommended after coloring of cuts across Kulchitsky to carry out them further by the Scorching method, i.e. to place in 0,25% water solution of potassium permanganate (on 20 — 30 sec.), to wash out a distilled water and to differentiate coloring in mix: oxalic to - they are 1 g, potassium sulfite — 1 g, a distilled water — 200 ml.


Bibliography: Merkulov G. A. Course of the patologogistologichesky equipment, page 217, L., 1969; Kultschitzky N. tiber die Farbung der markhaltigen Nervenfa-sern in den Schnitten des Zentralnerven-systems mit Hamatoxylin und mit Karmin, Anat. Anz., Bd 5, S. 519, 1890.


I. E. Hesinonim

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