KRISTELLERA METHOD

From Big Medical Encyclopedia

KRISTELLERA METHOD (E. Christeller, is mute. the pathologist, 1889 — 1928) — the way of preparation of frozen sections of the big sizes applied to studying of a gistotopografiya normal or patholologically the changed bodies.

It is offered by Kristeller in 1924. Cuts produce by means of a special little table for the big freezing microtome. Material for a research in the form of a plate 1 — 3 cm thick is fixed in solution of formalin within a day, then cut on more laminas (0,5 — 0,3 cm) and in addition condensed in formalin of 1 — 2 days. After washing a dist, water the plate is placed on a little table of the microtome and slowly (within 20 — 30 min.) expose to frost. The made cuts 15 — 30 microns thick straighten in flat cups with water, pull on glass of the corresponding size and dehydrate, applying alcohol on drops. Then glass with a cut is watered with divorced solution of photoxylin and dried up. The cut pasted thus is immersed in water together with glass, painted, washed out, dehydrated and concluded in Canada balsam. Drugs allow to judge with a certain relativity

about gistol, structure, and also the nature of distribution of defeats in body. See also Gistotopografichesky cuts .


Bibliography: Romeys B. Mikroskopicheskaya of the technician, the lane with it., page 125, M., 1953; Christeller E. Eine neue einfache Methode zur normalen und pathologischen Histotopographie der Organe, Virchows Arch. path. Anat., Bd 252, S. 783, 1924.

I. E. Hesinonim

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