KITTA-TAROTSTSI WEDNESDAY (Th. Kitt, is mute. veterinarian, sort. in 1858; G. Tarozzi, ital. physiologist, sort. in 1868) — a medium for cultivation of anaerobic microbes. Entered at the beginning of 20 century by Smith (Th. Smith) in bacterial, practice the hepatic mediums having recovery properties were used by Keith for cultivation of anaerobe bacterias. Especially hepatic environments were widely adopted after in 1905 Tarozzi considerably increased their recovery properties, adding pieces of a boiled liver to broth that allowed to use environments without use of special methods for removal of oxygen of air. Along with the brain circle of Gibler (E. von Hibler) To. — T. page played a big role in development of the doctrine about anaerobe bacterias.
Wednesday prepares as follows: the cow liver cut by pieces (1 — 2 cm) is weighed, filled in with triple amount of liver broth and 30 min. are boiled; then it is filtered for desludging. Pieces of a liver carefully wash out under the crane and display in test tubes on 3 — 4 g on 7 — 8 ml of the environment, will sterilize in the autoclave at 1 atm within 30 min. Before sterilization in test tubes pour a liquid paraffin (liquid paraffin) 0,5 — 1 cm of Wednesday high shall be transparent, without small dregs and vaguely long time can remain. Before the use it should be boiled for deaerating.
By further researches it was revealed that the same recovery properties also other animal fabrics and that for production have To. — T. the page can be used also a liver or muscles of any animal; it is important at the same time that they did not contain fat. Pieces of muscles can be replaced with the boiled meat passed via the meat grinder washed under the crane from small crumbs. Instead of liver broth for the environment K. — T. the page is used meat or the peptone broth prepared on Hottinger's repitch.
Mediums of type K. — T. pages are very different: instead of broth use ascitic liquid or blood serum, to-rymi fill in pieces of various bodies — a liver, a kidney, a brain, etc. They are applied to cultivation of various anaerobic microbes.
See also Mediums .
Bibliography: The guide to microbiological diagnosis of infectious diseases, under the editorship of K. I. Matveev, page 451, M., 1973; The Reference book on microbiological and virologic methods of a research, under the editorship of M. O. Birger, page 77, M., 1973; Kitt Th. Milzbrei-und Blut-breinahrwasser zur Ztichtung pathogener Anaerobier bei Luftzutritt, Zbl. Bakt., I. Abt. Orig., Bd 103, S. 42, 1927; Tarozzi G. Uber ein leicht in aero-ber Weise ausfiihrbares Kulturmittel von einigen bis jetzt fur strenge Anaeroben gehaltenen Keimen, ibid., Bd 38, S. 619, 1905.
A. M. Chelny.