KAYSERLING METHOD

From Big Medical Encyclopedia

KAYSERLING METHOD (To. Kaiserling, is mute. the pathologist, 1869 — 1942) — a way of consecutive fixing of anatomic drugs for the purpose of preservation of natural color of fabrics at long-term storage. It is offered in 1896. Transition of oxyhemoglobin of blood to a methemoglobin, and then to neutral hematin is the cornerstone of a method. The method provides three phases of processing of material.

1. The fresh, not washed from blood drug is immersed on 1 — 5 days in Kayserling No. 1 liquid — formalin of 200 ml at potassium nitrate (saltpeter) of 15 g, potassium acetate of 30 g, water of 1000 ml. At emergence of dirty-brown coloring of fabric drug is removed from liquid.

2. Without washing, slightly dry-through drug immerse on 1 — 2 day in Kayserling No. 2 liquid — 80 — 95% alcohol. At recovery of natural color of fabrics drug is removed from alcohol.

3. Dry-through drug is placed for final storage in Kayserling No. 3 liquid — glycerin of 200 — 350 ml, potassium acetate to 800 g, a distilled water of 1000 ml. There is a large number of modifications To. m, Braul's methods, Yoresa, Peak, Homann, Shor are most widespread.

See also Histologic methods of a research , Drugs anatomic .



Bibliography: Boguslavsky T. B. Production of topografo-anatomic drugs and technique of some anatomic researches, M., 1959; Romeys B. Mikroskopicheskaya of the technician, the lane with it., M., 1954; Residents of Yaroslavl B. M. Anatomicheskaya of the technician, Frunze, 1961; To an i vt ling C. Uber die Conservirung von Sammlungspraparaten mit Erhaltung der natiirlichen Farben, Berl. klin. Wschr., S. 775, 1896.

L. F. Gavrilov

Яндекс.Метрика