YaNUS (Janus) — group of the main aniline azoic dyes derivative of safranin. The name «yanus» arose on the association with two-faced Janus (in ancient Roman religion and mythology — god of doors, entrances and exits) founded on property of these dyes to change the color.
Enter into this group Janus green (diazine green), Janus blue (indazol) and Janus black (green Janus's mix with brown dye).
Janus apply hl. obr. for supravital coloring of mitochondrions (see); as the main dye it is used also for coloring of kernels, by identifications in fabrics of lipids, mycobacteria, etc. Dyes of this group are highly toxic; usually they are parted in 10 Ltd companies — 50 Ltd companies of times with isotonic salt solution.
Most widely apply Janus green. With the dried-up unstable culture of cells, a blood smear or marrow apply solution of dye on a slide plate. In 10 — 20 min. paint is merged, drug is rinsed with isotonic salt solution and covered with cover glass. Dye interacts with oxidizing enzymes of mitochondrions, and the last are painted in greenish-blue color. At recovery dye gains red color, and then becomes colourless. Apply mix Ya to simultaneous coloring of various organoids. green with neutral red (see); at the same time mitochondrions are painted in green color, and secretory granules and elements of a complex of Golgi — in red. Coloring quickly fades; for its fixing drug can be processed vapors of iodine and then to place in syrup of arabic gum.
For elective coloring of covers of bacteria the unstable smear within 5 — 10 min. is pickled in 1% solution phosphatomolybdic to - you and several seconds paint 0,1% Janus's solution green.
See also Histologic methods of a research.
Bibliography: Lille R. Patogistologicheskaya of the technician and a practical histochemistry, the lane with English, page 108, M., 1969.
Ya. E. Heein.