IMPREGNATION in histologic methods of a research (late lat. impraegnatio filling) — the special method of identification of structures of cells and fabrics based on their various ability to hold or recover salts of heavy metals (e.g., silver, lead, osmium, gold) which solutions impregnated objects of a mikroskopirovaniye in this connection their certain sites are painted in black, brown or other color.
The most important stage I. recovery of metal salt is, a cut it is more often caused by the reducing (recovering) effect of some substances which are contained in certain components of fabrics (ascorbic to - that, adrenaline, creatine, glutathione, some pigments and so forth). In some cases an object is processed the reducing reactants (formalin, pyrogallic to - that, etc.); sometimes as the reducing agents at And. use a sunlight or uv radiation from artificial sources, subpussycats a leniye, heating etc.
In process And. the sizes of intermicellar spaces of fabric elements, electrostatic properties of microscopic structures, the size of the formed particles of the recovered metal, etc. are of great importance. Therefore success And. substantially is defined by way of fixing, pH of the applied solutions, purity of the used reactants and other factors. Changing P.'s conditions, it is possible to reach selective identification of these or those fabric elements depending on research problems.
It is more deeply studied And. silver. Success of silvering in many respects depends on the corresponding microscopic and submicroscopic density of fabric elements and low contents in them protective colloids (see. Silverings methods ).
And. silver salts use for identification of nervous cells, neuroglial elements, peripheral nerve fibrils and their terminations (see. Bilshovsky-Groce-Lavrentiev method ), a reticular stroma of bodies, intercellular substance of an epithelium and smooth muscles, pale treponemas, etc.
V. V. Kupriyanov (1958) applied And. caustic silver of connective tissue covers for a research their circulatory and limf, vessels. On drugs there are visible many details of a structure of a vascular wall, including features of cellular elements. The technique offered E. I. Rasskazova (1954) for is the cornerstone of this modification And. nervous elements.
For the purpose of improvement of quality of drugs cuts the ambassador I. silver quite often tint salts of gold (see. Gilding methods ). Salt of gold can replace with saturated solution of hydrogen sulfide in a distilled water or 25% solution of sodium sulfide. Chloric and gold chloride is recovered in fats and rich with lipids, especially a myelin, sites of fabrics, painting them in violet color. From ways I. salts of gold kept value a method of identification of a neuroglia according to Ramone-and-Kakhalyu (1926) in various modifications, and also a number of methods I. myofibrils, neurofibrilla and nerve terminations.
And. serves as osmium (is more often osmium tetroxide) preferential for identification of fats and lipoid substances (are painted in olivkovocherny or brown tone). And. osmium lowers solubility of fats in absolute alcohol, chloroform, essential oils, etc. owing to what this method has advantage, especially during the studying of myelin nerve fibrils. And. osmium apply also to identification of a lamellar complex (Golgi's complex) in cells, and also to fixing of cells for the purpose of further submicroscopy (see).
See also Histologic methods of a research .
Bibliography: Morphological bases of microcirculation, under the editorship of V. V. Kupriyanov, page 20, M., 1965; Merkulov G. A. Course of the patologogistologichesky equipment, page 181, L., 1969, bibliogr.; P about m of e y with B. Mikroskopicheskaya of the technician, the lane with it., M., 1954, bibliogr.; P about with to and N G. I. and Levinson B. of JI. Microscopic equipment, page 211, M., 1957.
Ya. E. Hesinonim