HOLZER METHOD of COLOURING
(W. Holzer, Austrian neurologist, sort. in 1906) — the way applied in histology to identification of fibers of a neuroglia by coloring of drug crystal violet after pickle phosphatomolybdic to - that. It is offered in 1921 by Holzer.
On modern representations, selectivity of X. in m of the lake it is caused by specific binding by fibers of a neuroglia (see) phosphatomolybdic acid therefore the main trifenil-naftilmetanovy dyes (e.g., crystal violet) have an opportunity by means of the amino groups to join the pickled structures.
X. the m of the lake yields the best results during the fixing of material in 10% solution of formalin (see), twice divorced 96% alcohol. The fixed material is frozen, make cuts; except frozen also paraffin and celloidin sections are suitable (after removal of weight for filling). Cuts process 50% alcohol, during 30 — 90 sec. keep in mix 0,5% of water solution phosphatomolybdic to - you from 96% alcohol (1:3), dry on glass the filter paper moistened with mix of 100% of alcohol with chloroform (1:4) and cover 10% with solution of crystal violet in mix of 100% of alcohol with chloroform (1:4). Later 2 — 5 sec. the cut is washed by 10% solution of potassium bromide, dry filter paper, differentiate in the acidified mix of aniline oil with chloroform, process a xylol and conclude in balm. Fibers are painted in black-violet color; also kernels and partially cytoplasm of neyroglial-ny cells come to light.
See also Histologic methods of a research.
Bibliography: R about m e y with B. Mikroskopich
of Skye of the technician, the lane with it., page 445, M., 1954; Holzer W. Obereine neue Methode der Gliafaserfarbung, Z. ges. Neurol. Psychiat., Bd 69, S. 354, 1921. I. E. Hesinonim