HISTAMINE TEST — method of a research of acid-forming function of a stomach. It is applied most often to definition true achlorhydrias (see), however it can be used also for studying of a functional condition of gastric glands at the healthy person.
Gistamin (see) — fiziol, the mediator causing both nervous, and humoral excitement of gastric glands; is the strongest specific activator of gastric secretion, has preferential effect on obkladochny cells [B. P. Babkin, 1960; H. Bockus, 1963].
The histamine is entered subcutaneously; its action begins in 7 — 10 min. after introduction. The maximum secretory answer is shown in 20 — 30 min., reaction period of gastric glands apprx. 1 — 1,5 hour (Yu. I. Fishzon-Ryss, 1972). To of the item it is contraindicated to patients with the expressed changes of cardiovascular system, after recent bleeding, at allergic diseases, pregnancy, suspicion on existence of a pheochromocytoma. After an injection of a histamine quite often there is a feeling of heat, a headache, erubescence. These phenomena keep not for long; at their sharp expressiveness administer the antihistaminic drugs (see. Antihistaminic substances ).
There are several ways of definition of secretory function of a stomach by means of G. of the item.
Simple item. it is carried out on an empty stomach. After extraction of contents of a stomach a stylet the phosphate histamine is entered subcutaneously in a dose of 0,01 mg/kg, dihydrochloride of a histamine — in a dose of 0,08 mg on 10 kg of weight. Then within an hour, every 15 min. receive 4 portions of a gastric juice, in Krom determine by method of titration free salt to - that.
Double G. of the item. also it is carried out on an empty stomach. A histamine enter in the same dose, as at simple G. the item [Glass (V. of J. Glass), 1960], twice: right after gastric emptying by a stylet and in 30 min. after the first injection. After each introduction investigate two portions of a gastric juice at an interval of 15 min. Double G. allows to define the item not only a true achlorhydria, but also a ratio of functional and organic factors in disturbance of acid-forming function of a stomach.
Lamblen's way. It is offered by Lamblen (A. Lambling) with coauthors in 1953. By means of a stylet pump out contents of a stomach on an empty stomach (a portion And), within 15 min. constantly pump out contents (a portion In) and enter subcutaneously a muriatic or phosphate histamine at the rate of 0,1 mg on 10 kg of weight. After that during 2 hours every 15 min. pump out a gastric juice. In every portion determine the volume and acidity of the received contents. The quantity of the gastric juice which is allocated at healthy people during 2 hours after introduction of a histamine fluctuates from 150 to 250 ml. At diseases of a stomach this quantity can decrease or increase. The quantity salt is normal to - you make from 44 to 84 mekv/l. Figures St. 84 mekv/l characterize raised, lower than 44 mekv/l — the lowered acidity.
Maximum histamine test of Kay. It is carried out on an empty stomach. It is offered by Kay (A. Kau) in 1953. A phosphate histamine enter in a dose 0,04 mg/kg, and dihydrochloride of a histamine in a dose of 0,024 mg/kg. For 45 min. investigate basal secretion, then administer the antihistaminic drug (usually 2 ml of 2% of solution of Suprastinum) and within 30 min. collect a gastric juice. After this make an injection of a histamine in the specified dose then for an hour pump out a gastric juice. According to Kay, basal secretion salt to - you normal make 70 mg, after a maximum load a histamine — 422 mg, at a peptic ulcer of a duodenum — respectively 265 mg and 837 mg. Test allows to judge quite precisely degree of a mucosal atrophy of a stomach, however complexity of its carrying out and side reactions prevent widespread introduction of the test in a wedge, practice.
See also Stomach, methods of a research .
Bibliography: Babkin B. P. Secretory mechanism of digestive glands, page 240, L., 1960; L. I Haler. Gastric secretion and mechanisms of its regulation at the healthy person, page 9, L., 1975, bibliogr.; Fishzon-Ryss Yu. I. Modern methods of a research of gastric secretion, page 55, L., 1972; Bockus H.L. Gastroenterology, v. 1, p. 307, Philadelphia — L., 1963; Clea tor I. G. M. a. o-Comparison between the effect ofpentaga. strin and histalog on gastric acid secretion, Amer. J. dig. Dis., v. 17, p. 713, 1972, b b-liogr.; S with o b i e B. A. Histalog (Ameta-zole hydrochloride) as a substitute for histamine in determination of maximal acid output, Brit. med. J., v. 1, p. 1287, 1965.
S. I. Rapoport.