HADDLSONA REAKTION (I. F. Hudd-leson, amer. bacteriologist, sort. in
1893) — the agglutination test
on glass used for laboratory diagnosis of a brucellosis. Haddl-sonom is offered in 1926.
The method is based on property of the concentrated antigen and whole blood serum of pain-logo in the presence of electrolytes quickly to give a specific agglutination test (see). For statement of reaction and its account Hald Leon offered the special device representing a wooden box with an electric bulb inside. As a cover of a box serves glass, on Krom carry out reaction. Antigen (the killed suspension of brucellas) for reaction is prepared for 12% solution of sodium chloride, dyes gentsianovy violet or diamond blue are added to Krom. In 1943 E. I. Kaytmazova modified X. river, having simplified technology of its performance and a copy-book of preparation of antigen.
For statement of reaction the blood serum received in the usual way shall be transparent. Glass, on Krom carry out reaction, line everyone on 6 squares about 4X4 cm. On the first square write down number of ispytuyemy serum at the left, in the following squares spill not divorced ispytuyemy serum on 0,04; 0,02; 0,01; 0,02 ml; bring 0,03 ml of antigen in the last square. To the first three doses of serum add 0,03 ml of antigen, to the last dose (control of serum) — 0,03 ml of isotonic or 12% of solution of sodium chloride. For control of antigen add 0,03 ml of isotonic solution of sodium chloride to 0,03 ml of antigen. Serum is carefully mixed with antigen a glass rod, since the minimum dose of serum. Then glass is evenly warmed up over a flame of a spirit-lamp so that all surface of glass heated up approximately to t ° 37 ° (apprx. 2 min.). At positive reaction the first minutes visible flakes appear. The maximum term of observations is 8 min.
The accounting of results is made with the naked eye according to the following scheme: a full enlightenment of liquid with large or fine-grained flakes — 100% of agglutination (+ + + +); almost full enlightenment of liquid with obviously noticeable flakes — 75% of agglutination (+ + +); insignificant enlightenment of liquid with noticeable flakes — 50% of agglutination (+ +); muddy liquid with scarcely noticeable granularity (+); evenly muddy liquid (—). Reaction is considered positive at agglutination not less than on two pluses.
Results of X. rubles estimate as follows: lack of agglutination in all doses of serum demonstrates that reaction negative; agglutination in the first dose (0,04 ml) of serum — result doubtful; agglutination in the second or in the third dose (0,02; 0,01 ml) serums — a positive take; agglutination on four pluses in all doses of serum — result sharply positive.
For diagnosis of a brucellosis only positive takes of reaction matter. At doubtful result repeated carrying out reaction in 7 — 10 days and statement of an allergy test is recommended. At a negative take of reaction and existence of epidemiological and epizoologiche-sky indications repeated carrying out reaction is also recommended.
Positive X. the river is defined at the same time or slightly earlier positive reaction of Wright (see Wright reaction) and remains positive longer than the last.
In spite of the fact that X. the river serves only for qualitative test of agglutinins in blood of patients with a brucellosis, it gives big help at mass epidemiological inspections. In case of need definitions of a caption of agglutinins in the studied serums and at inspection of donors it is recommended to use Wright's reaction.
See also Brucellosis •, Serological researches.
Bibliography: A brucellosis, under the editorship of P. A. Ver-shilova, page 163, M., 1961; To and y t of m and-z about in and E. I. Bystry diagnostic method of a brucellosis at people, Zhurn. mikr., epid. and immun., No. 7-8, page 57, 1945; H u d d-leson I. F. a. Carlson E. K. A rapid method for performing the agglutination test in the serum diagnosis of Bang’s abortion disease in cattle, J. Amer. vet. med. Ass., v. 70, p. 229, 1926.
E. I. Kaytmazova.