GOLGI METHOD

From Big Medical Encyclopedia

GOLGI METHOD ( S. Golgi , the ital. histologist, 1844 — 1926) — the way of impregnation of bodies and fabrics applied in histologic researches of hl. obr. for identification of nervous cells and their shoots, at Krom an object is processed at first solution of potassium bichromate, and then solution of silver salt (or corrosive sublimate). It is offered in 1873. At this way of processing on the impregnated structures the fine-grained deposit of compounds of chrome with silver drops out (or mercury). Nervous cells and their shoots get black coloring after processing. There are tens of various modifications of G. of m. Good and bystry results are yielded by the simplest modification of Byubenet (1929), on a cut an object:

1) fix in 10% formalin (1 — 2 day);

2) chromeplate 2 days in 2,5% solution of potassium bichromate (at t ° 34 °);

3) drain filter paper, quickly rinse 2% AgNO solution 3 and then place in the same solution on 1 — 2 day (at t ° 34 °);

4) fill in in paraffin and produce cuts.

On Baginsky's way (1957) after fixing by formalin an object:

1) place for 3 — 5 days in 5% AgNO solution 3 , ammonium bichromate (at t ° 33 — 37 °);

2) drain filter paper, place for 10 — 15 min. in 0,5% solution, transfer to 1,5% AgNO3 solution on 1 — 7 days depending on research objectives (trial cuts);

3) wash out 6 — 10 hours in a dist, water.

The cuts made on the freezing microtome maintain 24 hours in 70% alcohol for removal of traces of silver, dehydrate, carry out through benzene and conclude in dense balm (without cover glass).

See also Silverings methods .

G. I Roskin

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