GISTOTOPOGRAFICHESKY CUTS

From Big Medical Encyclopedia

GISTOTOPOGRAFICHESKY CUTS (Greek histos a column, fabric + topos the place + grapho to write, represent) — the microscopic sections which are carried out through all body or body parts for the purpose of studying of their gistotopografiya are normal or pathologies. The way of preparation of G. of page is offered in 1924 it. pathologist E. Christeller. In the late twenties V. G. Shtefko one of the first in the USSR applied G. of page to studying of pulmonary tuberculosis. Broad application of G. of page was found in topographical anatomy and embryology. In pathological anatomy the method G. of page allows to judge localization of the center of defeat in bodies (e.g., an arrangement of a tumor, a cavity, etc.).

the Gistotopografichesky cut through a cavernous venous sine, the Turkish saddle and a hypophysis (the left half of an average cranial pole) of the person: 1 — hypophysis; 2 — a side surface of the Turkish saddle; 3 — dura mater; 4 — n. ophthalmicus; 5 — n. abducens; 6 — sinus cavernosus; 7 — a. carotis interna (coloring of no of Van-Gizona).

The principle of a method consists that the body (entirely) or the site of a part of a body is subjected to freezing, fill in in photoxylin or paraffin then do cuts (fig.) 20 — 40 microns thick on the big microtome. If the body has the big sizes (more than 15 cm), it is cut on pieces and give cuts from each of them. Marking of pieces of body and cuts from them allows to make reconstruction of body an arrangement of all cuts one after another on one glass.

Coloring of cuts, depending on research objectives, is made by various histologic methods (according to Van-Gizona, etc.). By means of special techniques of coloring or impregnation the reticular stroma, collagenic and elastic fibers, nervous both other fabrics and their ratio come to light. After G.'s coloring by the village conclude on Wednesdays by usual methods, filling in plastic is recommended (see. Histologic methods of a research ). For the purpose of studying of topography of an object use a binocular magnifying glass or small increase in a microscope (about. 7kh), and for detection of features of a structure of fabrics — big increase in a microscope.


Bibliography Merkulov G. A. Course of the pathological equipment, page 111, L., 1969; Christeller E. Eine neue einfache Methode zur normalen und pathologischen Histotopographie der Organe, Virchows Arch. path. Anat., Bd 252, S. 783, 1924.

S. S. Mikhaylov.

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