GINSA METHOD

From Big Medical Encyclopedia

GINSA METHOD (The N of A. Gins, is mute. bacteriologist, sort. in 1883) — a way of identification of capsules of bacteria by means of processing of drug ink and the subsequent coloring by thionine.

The technician of a method

the Drop of the studied culture is pounded in a drop of the ink divorced a dist, water concerning 1: 1. From mix prepare a smear as a blood smear. The smear is fixed within 1 min. in saturated solution of corrosive sublimate: 5% solution of corrosive sublimate in boiling a dist, water remove from fire and cool in the dark place until long crystals drop out in a deposit (if solution is cooled on light, then small crystals which do liquid muddy and of little use for work drop out). The transparent solution of corrosive sublimate sated in the cold is merged and used for fixing. Well wash out drug water. The smear is painted carbolic thionine within 5 — 10 min., washed out water, dried up, concluded in Canada balsam or fir-seed oil and covered with cover glass 0,17 mm thick. Investigate dry system or an immersion objective 90; a background — smoky-brown. Capsules of bacteria are not painted and are visible as white auras around the bacteria painted in violet color.

See also Burri a method , Colouring of microorganisms .


Bibliography: The reference book on microbiological and virologic methods of a research, under the editorship of M. O. Birger, page 27, M., 1973; Gins H. A. Zur Technik und Verwendbarkeit des Burrischen Tuschever-fahrens, Zbl. Bakt., I Abt. Orig.,

Bd 52, S. 620, 1909,

M. A. Peshkov.

Яндекс.Метрика