GILDING METHODS

From Big Medical Encyclopedia

GILDING METHODS — the ways of histologic processing of fabrics salts of gold used preferential for studying of nervous and muscular tissue. In gistol, the equipment 3. m usually apply to toning of drugs, impregnirovanny silver, use independent methods of impregnation by gold less often (see. Impregnation ).

For gilding use 0,5 — 1% solution of chloric gold (AuCl 3 ) or zolotokhloristovodorodny to - you are H (AuCl 4 ) - 4H 2 O. During the toning of cuts, impregnirovanny silver (see. Silverings methods ), weakening of impregnation of fine nerve fibrils and synoptic structures can be noted a nek-swarm. Cuts place in divorced solution of chloric gold (usually several drops of 1% of solution for 10 ml a dist, waters, at some modifications apply more konts. solutions — to 1%) it is frequent with addition of a small amount acetic to - you. Cuts from brownish-yellow become gray and steel or reddish-violet; impregnirovanny silver elements at the same time gain black or dark-violet color (see. Bilshovsky-Groce-Lavrentiev method ).

Impregnation by gold is applied to identification of nervous cells with their shoots and the terminations, elements of a neuroglia, connective tissue fibers, and also myofibrils and so forth (see. Ramón-and-Kakhalya methods , Ranvye methods ).

For identification of connecting fabric in c. N of page apply Biondi's way: the cuts made by a freeze-etch method pickle in solution of potassium permanganate then they are processed mix of potassium sulfate with oxalic to - that and gild in mix of chloric gold with corrosive sublimate. All manipulations during the work with chloric gold should be made purely washed up glass needles.

See also Histologic methods of a research .


Bibliography: Merkulov G. A. Course of the patologogistologichesky equipment, page 185, etc., L., 1969; P about m of e y with B. Mikroskopicheskaya of the technician, the lane with it., page 465, M., 1954.

Ya. E. Hesinonim

Яндекс.Метрика