GARRISON TEST (G. And. Harrison; Garrison's synonym — Fouchier test) — qualitative test on bilirubin in urine. It is based on oxidation bilirubin (see) to biliverdin at interaction with Fouchier's reactant [25 g trichloroacetic to - you, 10 ml of 10% of solution of polutorakhloristy iron (FeCl 3 ) and 100 ml dist, waters].
Normal practically does not contain in urine of bilirubin. Emergence in urine over 0,07% of bilirubin is a serious diagnostic character and testifies to gepatobiliarny pathology (mechanical jaundice, infectious hepatitis, etc.).
Technology of definition
add a half of its volume (5 ml) To 10 ml of urine konts. solution of barium chloride (BaCl 2 ), mix and filter via the paper filter. Then apply 2 — 3 drops of a reactant of Fouchier on the middle of the straightened filter. Emergence of blue or green coloring demonstrates presence at urine of bilirubin.
In the modified option of test of Hokinson, Watson, Turner (V. Hawkinson, Page J. Watson, R. H. Turner, 1945) use the tape of filter paper which is previously impregnated konts. BaCl solution 2 . The tape is lowered for 1 min. in urine, presence of bilirubin in a cut is wanted to be defined, then dried and put Fouchier's reactant. Positive reaction is confirmed by emergence of blue-green coloring.
, the item is one of the most sensitive qualitative tests on bilirubin. Sensitivity of test makes from 0,5 to 1,7 mg of % (according to different authors) - G. is offered the item as the unified test for qualitative test of bilirubin in urine.
See also Hyperbilirubinemia .
Bibliography: The reference book on clinical functional trials, under the editorship of A. Gitter and L. Heylmeyer, lane with it., page 314, M., 1966; Harrison G. And. Chemical methods in clinical medicine, L., 1947.
V. Ya. Maximov.