ERLIHA-BIONDI COLOURING (P. Ehrlich, is mute. doctor, bacteriologist and biochemist, 1854 — 1915; A. Biondi, the ital. pathologist, 1846 — 1917) — a way of multi-color coloring of blood smears and tissue specimens.
Coloring is made triatsidy, the offered P. Ehrlich in 1891, in Biondi's modification. Triatsid represents neutral mix of saturated water solutions: 10 ml orange (see. Orange),
1 ml of magenta acid (see) and 3 ml of imperial green (see). A. Pappenheim in 1901 suggested to add in triatsid Ehrlich — Biondi saturated solution methylene blue (see) that improves coloring of kernels of cells (see A. Pappenheim methods).
Smears fix dry heat, pieces of fabric before paraffin embedding fix in mix Carnoy (see Carnoy liquid) or in the fixers containing corrosive sublimate; almost any fixing of material is suitable for frozen sections. Before coloring paraffin (or photoxylin) is deleted by means of a xylol or toluene. Coloring lasts apprx. 10 min. then drug is rinsed by 0,5% with solution acetic to - you and differentiate (weaken) coloring of 70% alcohol.
The microscopy of smears can be made after drying; cuts dehydrate, clarify and conclude in balm. As a result of E. — B. lakes chromatin of kernels, and also slime and the main substance of a cartilage gain blue-green color, kernels, cytoplasm, fibers of connecting fabric are painted in red color of different shades, erythrocytes — in orange, granules of neutrophilic leukocytes — in reddish-violet, eosinophilic granules — in bright red color. Granules of basophiles, mast cells and cytoplasm of lymphocytes usually remain uncolored.
See also Histologic methods of a research.
Bibliography: R about m e y with B. Mikroskopi
the chesky equipment, the lane with it., page 172, M., 1954; Ehrlich P. Farbenanalytische Untersuchungen zur Histologie und Klinik des Blutes, B., 1891. I. E. Hesinonim