CLONE

From Big Medical Encyclopedia

CLONE (Greek. klon escape, the offspring) — set (population) of the cells (or unicells) which resulted from vegetative reproduction of one stem cell (unicell).

During the receiving To. (so-called cloning) make disaggregation of fabric by means of proteolytic enzymes, crops of a cellular suspension on fluid or solid artificial nutrient mediums, the subsequent allocation of single cells, originative of one cell owing to difficulty to create to K. Potomstvo stable culture conditions of cells of in vitro not always is genetically homogeneous.

In virology the cloning is the cornerstone of receiving genetically homogeneous lines of viruses. For this purpose use methods of end-point dilution, receiving To. viruses from one, infected cell etc.

The cloning is widely applied to studying of a number of theoretical and applied problems of experimental biology and medicine, napr, oncology (see), immunogenetics (see), geneticists of somatic cells (see), at a research of linkage groups, during the mapping of chromosomes etc. See also Cultures of cells and fabrics .



Bibliography: Dubinin N. P. General genetics, M., 1976, bibliogr.; Lebedeva M. N. Microbiology, M., 1969; Petrov R. V. Immunology and immunogenetics, M., 1976, bibliogr.; Paul Dzh. Culture of cells and fabric, the lane with English, M., 1963; T e r c and M. Genetika and a zooblast, the lane with English, M., 1977.


Yu. E. Ershikova.

Яндекс.Метрика