BUTANOLEKSTRAGIRUYEMY IODINE — iodine of biologically active tironin of blood serum (thyroxine and triiodothyronine). It is applied as the test to diagnosis of diseases of a thyroid gland (see. Craw diffusion toxic , Hypothyroidism ). Technology of definition consists of the following processes: extraction of the iodated amino acids from blood serum in acid medium of N butanol; washing of butanolovy extract from the iodated tyrosines and inorganic iodine an alkaline reactant (4 N the NaOH solution containing 5% of Na 2 CO 3 ); release of elementary iodine from butanolovy extract distillation, dry ashing or oxidizing eliminating; quantitative definition of iodine (titrimetric, colorimetric or radio activation analysis).
Technique: 0,5 ml of blood serum bring in a test tube of 8 — 10 ml with a ground stopper. To serum add 0,1 ml of 10% of solution H 2 SO 4 and 4 ml of N butanol. Stir up 1 min. Centrifuge 10 — 15 min. at 2500 — 3000 rpm. Butanolovy extract is transferred to a dividing funnel with a capacity of 50 — 60 ml. The rest of serum is extracted once again as it is described above.
The integrated butanolovy extracts wash out an alkaline reactant for what in a dividing funnel add 8 ml of an alkaline reactant, stir up within 5 min. on the shaking machine. Dividing funnels place vertically in a support and 10 min. for division of water and butanolovy phases allow to be defended. The alkaline layer is merged. Washing is carried out by two more times as it is described above. After removal of traces of caustic solution in a funnel flow 4 ml 0,005 N of solution of cerium sulfate (prepare it cultivation of stock solution of cerium of 0,35 N of H 2 SO 4 ; stock solution is prepared by dissolution of 10,1 g of cerium of sulfate solution H, oxide in 250 ml 5 N 2 SO 4 ). Funnels stir up 30 min. The layer of cerium is merged. The Butanolovy phase is washed out by 8 ml of water during the stirring within 5 min. In 20 min. upholding the aqueous phase is discarded. Bring 1 ml of 0,03% of solution of potassium bromide and 4 ml 0,25 N of NaOH in butanolovy extract. Stir up 15 min. and after upholding within 30 min. transfer a water layer to test tubes in which carry out reaction for colorimetry.
Colorimetry: add 3 ml 0,3 N of solution of arsenite to each test tube (prepare: 7,42 g arsenous to - you dissolve in 250 ml of the bidistilled water, add 14 ml konts. H 2 SO 4 also bring the bidistilled water to 500 ml; at dissolution warm up, without bringing to boiling). Tests place in the water bath with t ° 42 °. Place in a bath also a test tube from 0,01 N solution of cerium in the quantity sufficient for a series of definitions (prepare dilution of stock solution of cerium 10% solution H 2 SO 4 concerning 1: 10). In 10 min. in each test tube add 1 ml 0,01 N of solution of cerium so that to fotometrirovat contents of each test tube in 30 min. Colorimetry is carried out at the wavelength of 400 nanometers against a distilled water. The amount of thyroxine and triiodothyronine is determined by the standard curve constructed taking into account additive of thyroxine to serum in concentration 0,0; 0,02; 0,04; 0,08; 0,16 mkg with after-treatment of serum as it is described above.
The amount of thyroxine and triiodothyronine at healthy makes 3,0 — 7,5 mkg of %, at primary hypothyroidism to 3,0 mkg of %. at a thyrotoxicosis — 7,5 — 28,0 mkg of %.
Bibliography: V. G. and Nikolayenko's rams Η. T. Diseases of a thyroid gland, Mnogotomn, the management on vnutr. Bol., under the editorship of Ε. M. Tarseva, t. 7, page 23, L., 1966; r about l of l of m and and And. Clinical endocrinology and its physiological bases, the lane with English, M., 1969, bibliogr.; P about s n e of 1. A simplified procedure for the determination of butanol-extractable iodide in serum or plasma, J. Lab. clin. Med., v. 57, p. 314, 1961.
G. S. Stepanov.