BRAIDS WAYS

From Big Medical Encyclopedia

BRAIDS WAYS (G. Magyary-Kossa, the Hungarian pharmacologist, 1865 — 1944) — histologic methods of identification of lime (calcium and calcium phosphate) in fabrics. Pieces of fabric fix in alcohol and after incomplete decalcifications (see) or without it prepare cuts.

Braids way I — identification of lime by means of pyrogallic to - you: cuts of fabric for 5 min. place in 2,5% solution pyrogallic to - you, 1,25% of caustic soda then carefully wash out water are added to Krom, dehydrate and conclude in balm. Lime is painted in brown color.

Braids way II — identification of lime by means of caustic silver: the cuts taken from a distilled water place AgNO solution in 3 — 5% 3 for 10 — 60 min., wash out a distilled water, postpone to 1 — 2 min. in 5% solution of sodium hyposulphite, wash out water. dehydrate, clarify and conclude in balm. Deposits of phosphate lime are painted in black color.

For differential identification of calcium and calcium carbonate Eryosh (G. Eros, 1928) suggested to immerse cuts of fabric after coloring on To. page II in 1% the solution of magenta acid saturated in the cold with potash alum then to differentiate in 3% muriatic alcohol. Deposits of calcium carbonate are painted at the same time in red color.

See also Histologic methods of a research .



Bibliography: Merkulov G. A. Course of the patologogistologichesky equipment, page 288, L., 1969; P about m of e y with B. Mikroskopicheskaya of the technician, the lane with it., page 387, M., 1954; To s s a J. tiber die im Organismus kunst-lich erzeugbaren Verkalkungen, Beitr. path. Anat., Bd 29, S. 163, 1901.

I. E. Hesinonim

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