BERG METHOD (W. N. Berg) — a method of identification milk to - you in a gastric juice. It is described in 1912.
Qualitative test milk to - you in a gastric juice (Berg's reaction) is based on formation of bright yellow coloring at interaction of a reactant of Berg (2 drops konts. salt to - you plus 2 drops of 30% of ferric chloride for 100 ml of the diart. of water) with milk to - that.
Emergence of lactic acid in a gastric juice quite often accompanies development of malignant new growths of a stomach. Therefore at a research of a gastric juice it is necessary to define also lactic acid.
Technology of definition
In a volumetric flask from 100 ml of the diart. of water flow several drops of a reactant of Berg. In two test tubes pour 10 ml of the received solution and add to one of them on drops the filtered gastric juice (only 5 — 6 drops). In the presence of milk to - you contents of a test tube are painted in bright yellow color. For control in other test tube it is possible to add 2 — 3 drops of solution milk to - you.
Exists modification Berg's reactions, on a cut instead of a pure gastric juice use its radio extract. Test with a radio extract is the most sensitive and allows to define 0,05% konts. milk to - you. In this case solution gets slightly greenish coloring, 0,1% solution give flavovirent coloring.
Bibliography: Pearce E. A histochemistry, the lane with English. M, 1962; The Guide to Kleene ~ to chesky laboratory researches, under the editorship of B. A. Kost and L. G. Smirnova, page 557, M., 1964; The Reference book on clinical laboratory trials, under the editorship of. K A. Kost, page 225, M., 1968.
V. Ya. Maximov.