BACKGROUND COLOURINGS — the name of processing of tissue specimens the acid dyes diffuzno impregnating drug and creating a contrast background for kernels and other structures painted by the main dye. The term is used also for designation of the paints applied at this processing.
By means of F. lakes reveal preferential cytoplasmatic structures of cells and various intercellular substances. As acid dyes for F. lakes most often apply eosine, magenta acid, a Bordeaux, orange (red tone), indigo carmine (blue), picric to - that (yellow). Selection of dye for F. the lake usually is defined by color and properties of the main dye. If coloring of drug by the main and background dyes is made consistently, in the separate painting solutions, names of dyes can be connected the union «and», napr, coloring by hematoxylin and eosine. If both dyes form uniform solution and coloring of basphilic and oksnfil-ny structures of drug make at the same time, names of dyes connect a hyphen, napr, coloring by azur-eosine.
In addition to pure solutions of dyes, for F. lakes often use mix of two or several acid dyes, to-rye differ on affinity to certain structures and can paint different fabric elements, napr' mix picric to - you with magenta acid — the pikrofuk-synonym is based On it a number of ways of multiple coloring gistol. drugs, napr, according to Van-Gizona (see Van-Gizona a method).
Concept F. the lake is considerably conditional since acid dyes are to some extent capable to paint kernels of cells, and the main paint not only kernels, but also salts of calcium, slime, the main substance of cartilaginous tissue. At certain ways of processing of drug the main dye (e.g., iron hematoxylin on Geyden-gayna) accurately reveals not only nuclear, but also cytoplasmatic structures.
See also Histologic methods of a research.
Bibliography: Merkulov G. A. Course
of the patologogistologichesky equipment, page 121, L., 1969. Ya. E. Hesinonim