ADENOASSOTSIIROVANNY VIRUSES

From Big Medical Encyclopedia

ADENOASSOTSIIROVANNY VIRUSES (Greek aden — gland and armor. associare — to connect; synonym: adenosatellitny, adenoassotsiirovanny satellite viruses) — the small DNA-containing viruses breeding only in the presence of adenoviruses; are a part of group of parvoviruses.

Are for the first time described by the Italian virologists Arketti and Bochcharelli (1963).

And. are found in cultures of the adenoviruses (see) isolated from people and animals century.

Particle size A. century of 22 — 24 nanometers, they have cubic symmetry of ikosaedralny type. Their weight 7,5 * 10 6 dalton. The floating density of the majority of particles in an isopycnic gradient of chloride caesium makes from 1,38 (types 1 — 3 and H7) up to 1,43 g/cm 3 (type 4). Besides, there are more dense particles which diameter is 12% less, their floating density makes from 1,43 (X7 virus) to 1,47 g/cm 3 (And. century type 3). In cultures empty capsids with a density of 1,33 — 1,36 g/cm are also found 3 .

Assume that a capsid And. consists of 12 capsomeres century. Three structural proteins about a pier are a part of virion. it is powerful apprx. 66 thousand, 80 thousand and 92 thousand dalton. Adenoassotsiirovanny viruses of 4 type and the X7 virus have hemagglutinin. The first of them most intensively sticks together erythrocytes of the person of group 0 at t ° 4 °, and the second — erythrocytes of the person, a Guinea pig and a mouse. At t ° 37 ° elution of a virus without destruction of receptors of an erythrocyte is observed.

And. contain one-filamentous DNA about a pier century. weighing 1,5 — 1,7 * 10 6 dalton. It is the share of it from 18,9 (type 1) to 26,5% (type 4) of weight of virion, and guanine-tsitozin makes from 53% (And. century of type 2) to 58 — 62% (type 4) of weight of DNA.

Population And. consists of virions of two types differing on structure of the DNA thread which is their part century. Both types of DNA are complementary each other: one virions contain «plus» - thread, others — «minus» - thread. There are distinctions in their chemical structure: «plus» - threads contain 21,7% of thymidine, and «minus» - threads — 26,5%. Apparently, in the infected cells only «minus» - threads serve as matrixes for a transcription of genetic information And. century.

And. are very steady against heating century: at t ° 80 ° they are inactivated in 30 min., and at t ° 60 ° — only in 4 hours. They do not change within 30 days at t ° 25 ° and 6 months — at t ° 2 — 4 °. Are steady against effect of ether. Are inactivated by ultraviolet rays.

Several serological types A are known. century. Occur at primacies And. century of four types: people have types 1, 2, 3, at monkeys — types 1, 2, 3, 4. Types 2 and 3 have nek-paradise an antigenic community. Are found And. century also at cattle (X7 virus), pigs, dogs and hens. Clinically expressed diseases And. century, apparently, do not cause. They are quite often allocated together with adenoviruses from pharyngeal and rectal washouts at people at adenoviral diseases (see). Antibodies to And. century of types 1 — 3 are found in serums of 20 — 50% of people; infection happens usually in the early childhood. Even more widely these viruses are widespread among monkeys (macaques Rhesus factors and green African monkeys). The X7 virus, in addition to cattle, maybe, it is obvious, to infect people as their corresponding antibodies are found.

And. are defective viruses century, i.e. can breed only in the presence of «assistants» — adenoviruses. They have no specificity concerning «assistants», to-rymi there can be any adenoviruses of the person, monkeys, etc.

And. breed century in those cellular cultures where also their «assistants» — adenoviruses. Place of a reproduction And. century kernels of cells where virus particles sometimes create crystals are. Between separate types A. century, napr, between types 1 and 4, the interference can be observed (see the Interference of viruses). Quantity of particles And. century in culture usually in tens of times exceeds number of virions of adenovirus. In the course of a reproduction And. century considerably suppress reproduction of adonovirus - «assistants» in cellular cultures, and also ability of oncogenous strains (e.g., adenovirus of the person, type 12) to induce tumors at the Syrian hamsters. At the same time And. do not influence weight of a current at children of the respiratory diseases caused by adenoviruses century.

In a nek-swarm of degree function of «assistants» to reproduction And. can carry out herpetic viruses of the person and animals century (causative agents of a herpes simplex, chicken pox, cytomegaly of the person, Epstayn's virus — Burra and the causative agent of infectious rhinotracheitis of cattle). In this case only infectious DNA of an adenoassotsiirovanny virus and its fibrous casing (capsid), but connection of these components with formation of the virus particles capable to a reproduction is synthesized, does not occur.

Existence And. decides most often on the help of a submicroscopy century, also RSK, precipitation in gel and processing of the infected cells is used by fluorescent immune serums. And. it is possible to reveal century of type 4 and H7 on their ability to agglutinate erythrocytes.

See also Parvoviruses .

Bibliography: Blacklow N. R., Hoggan M. D. and. McCLanahan M. S. Adenovirus-associated viruses, Proc. Soc. exp. Biol. (N. Y.), v. 134, p. 952, 1070; Blacklow N. R. a. o. A seroepidemio-logic study of adenovirus-associated virus infection in infants and children, Amer. J. Epidemiol., r. 94, p. 359, 1971; In about u-cher D. W., Mel nick J. L. a. Mayor H. D. Nonencapsi dated infectious DNA of adeno-satelllte virus in cells coinfected with herpesvirus, Science, v. 173, p. 1243, 1971; Hogg an M. D. Adenovirus associated viruses, in book: Progr. med. Virol., ed. by J. L. Melnick, p. 211, MQn-chen — N. Y., 1970; Johnson F. B., Ozer H. L. a. H about g g a n M. D. Structural proteins of adenovirus-associated virus type 3, J. Virol., v. 8, p. 860, 1971; T orpl er G., D’ H a 1 1 ui n J. - C. a. Boulanger P. Electron microscopic observations of KB cells infected with adeno-associated satellite virus, J. Microec. (Paris), v. 11, p. 259, 1971.

AA. P. To a saw.

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