ADAMKEVICH REACTION

From Big Medical Encyclopedia

ADAMKEVICH REACTION (And. Adamkiewicz, 1850 — 1921, the Austrian doctor) — one of staining (high-quality) reactions on protein. It is caused by existence in protein of amino acid — tryptophane (see), forming with glyoxylic to - that the painted complexes. At addition konts. a chamois to - you to solution of protein and glyoxylic to - you on border of contact of liquids appear a red-violet ring.

Technology of definition. In a test tube pour 1 — 2 ml of the studied pas of squirrels of solution, add 1 ml konts. acetic to - you (always in the form of impurity contains in it glyoxylic to - that) and, having strongly inclined a test tube, carefully on a wall underlay apprx. 1 ml konts. a chamois to - you so that both liquids did not mix up. During the standing on border of two liquids the red-violet ring which is gradually extending to all liquid is formed.

Sensitivity of reaction apprx. 1: 200 000, and in the presence of copper ions increases to 1: 1 000 000; hydrogen peroxide suppresses reaction. It is necessary to remember that, in addition to tryptophane, the indole and its derivatives give this reaction.

Thanks to high sensitivity And. the river can be used for detection of protein in urine.

See also Gopkinsa methods .


Bibliography: Block P. and Bolling D. Amino-acid composition of proteins and foodstuff, the lane with English, M., 1949; Zbarsky B. I., Ivanov I. I. and Markhashev S. R. Biological chemistry, page 32, L., 1972.

V. Ya. Maximov.

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